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Diagnostic tool based on an HTLV-1-Tax expression system in eukaryotic cells using a poxvirus vector Souza, Jaqueline Gontijo de Fonseca, Flávio Guimarães da Martins Filho, Olindo Assis Carvalho, Andreateixeira Martins, Camila Pacheco Silveira Carvalho, Luciana Debortoli Reis, Jordana Grazziela Alves Coelho dos Giph Stancioli, Edel Figueiredo Barbosa HTLV-1 HAM/TSP Vacina ORGANISMOS [B]::Vírus::Vírus de RNA::Retroviridae::Deltaretrovirus::Vírus Linfotrópico T Tipo 1 de Primatas::Vírus Linfotrópico T Tipo 1 Humano COMPOSTOS QUÍMICOS E DROGAS [D]::Misturas Complexas::Produtos Biológicos::Vacinas 03 Saúde e Bem-Estar Human T-lymphotropic virus 1 (HTLV-1) induces an immune-mediated inflammatory disease affecting the nervous system that eventually is accompanied by ocular, rheumatic and dermatologic manifestations (HTLV-1 associated myelopathy/tropical spastic paraparesis, or HAM/TSP). Proviral load and HTLV-1 protein expression, mainly of Tax, is correlated with disease progression and induction of host–virus equilibrium breakdown that, reportedly, involves the presence of Tax-specific cytotoxic T lymphocytes (CTL), T regulatory cells and anti-Tax antibodies. Based on knowledge of anti-Tax antibodies as markers of disease progression, the objectives of this study were both to design an infection/transfection system using the Vaccinia virus and a tax-encoding plasmid for the expression of Tax protein as well as to use this cell support to evaluate anti-Tax IgG by flow cytometry. The flow cytometry assay was standardized using pooled sera from each test group (negative, asymptomatic and HAM/TSP patients). The HAM/TSP group presented higher IgG anti-Tax reactivity (above 70%) than the asymptomatic group (nearly 40% reactivity). The data indicate that the infection/transfection system is useful for assessing Tax expression. This is a promising assay for use as a diagnostic tool to detect IgG anti-Tax and monitor HTLV-1 infected individuals. 2025-01-01 2018-07-30T19:19:31Z 2018-07-30T19:19:31Z 2010 journal article SOUZA, Jaqueline Gontijode et al. Diagnostic tool based on an HTLV-1-Tax expression system in eukaryotic cells using a poxvirus vector. Journal of Virological Methods, v. 166, n. 1–2, p. 65-71, 2010. 0166-0934 https://arca.fiocruz.br/handle/icict/27728 10.1016/j.jviromet.2010.02.021 eng restricted access application/pdf Elsevier Ltd.