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oai:arca.fiocruz.br:icict/722372026-10-19T21:52:28Zcom_icict_3802col_icict_9180

Efficient cell model for assessing inflammatory responsive genes in Mycobacterium tuberculosis and SARS-CoV-2 co-infection Lucena, Thays Maria Costa de Miranda, Débora Elienai de Oliveira Arcoverde, Juliana Vieira de Barros Cavalcanti, Mariana Souza Bezerra Dantas, Willyenne Marília Pena, Lindomar José Lorena, Virginia Maria Barros de Rabello, Michelle Christiane da Silva Silva, Jaqueline de Azevêdo DOENÇAS [C]::Infecções::Infecções Bacterianas e Micoses::Infecções Bacterianas::Infecções por Bactérias Gram-Positivas::Infecções por Actinomycetales::Infecções por Mycobacterium::Tuberculose DOENÇAS [C]::Infecções::Infecções Respiratórias::Pneumonia::Pneumonia Viral::COVID-19 This work was supported by the Brazilian funding agencies: Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) - Grant Numbers: 140437/2022-3, 312366/2021-3, and 403844/2021-5 (Universal) and Fundação de Amparo à Ciência e Tecnologia de Pernambuco (FACEPE) - Grant Number: APQ-1119-2.02/22. Mycobacterium tuberculosis (Mtb) and severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) may induce immunopathology with extensive lung damage in hosts. To elucidate the dynamics of co-infection Mtb and SARS-CoV-2 and its impact on inflammatory mediators' expression, we conducted a study to evaluate A549, lung epithelial cells, as a potential model for hosting both pathogens simultaneously. Cell infection initiated with Mtb H37Rv and following a 24-h incubation period, the cells were then infected with SARS-CoV-2. After a 72 h incubation period, a precision test was conducted for both pathogens, and total RNA was extracted for subsequent analysis of gene expression by RT-qPCR of the target genes: IFN-γ, TNF-α, IL-6, and IL-1β. Additionally, the levels of IL-1β, IL-2, IL-4, IL-6, IL-10, IFN-γ, and TNF-α in the culture supernatants were measured. A549 cells are a stable and reliable cellular model for co-infection between Mycobacterium tuberculosis and SARS-CoV-2. Co-infection with both pathogens led to downregulation of IFN-γ, TNF-α, and IL-10, and upregulation of IL-6 and IL-1β compared to uninfected cells. A549 cells function as a cellular model for co-infection and seems a good model for elucidating host inflammatory responses in the initial site of infection. 2025-09-23T16:50:26Z 2025-09 journal article LUCENA, Thays Maria Costa de et al. Efficient cell model for assessing inflammatory responsive genes in Mycobacterium tuberculosis and SARS-CoV-2 co-infection. Tuberculosis, v. 154, n. 2025, p. 1-10, Sept. 2025. 1472-9792 https://arca.fiocruz.br/handle/icict/72237 10.1016/j.tube.2025.102672 1873-281X eng Tuberculosis restricted access application/pdf Elsevier