2026-10-25T15:48:49Zhttps://api.arca.fiocruz.br/oai/requestoai:arca.fiocruz.br:icict/747072026-10-17T19:21:41Zcom_icict_3802col_icict_9180
Comparison of DNA extraction methods for COVID-19 host genetics studies
Silva, Ronaldo Celerino da
Lima, Suelen Cristina de
Reis, Wendell Palôma Maria dos Santos
Magalhães, Jurandy Júnior Ferraz de
Oliveira, Ronaldo Nascimento de
Rathi, Brijesh
Kohl, Alain
Bezerra, Marcos André Cavalcanti
Pena, Lindomar
DNA
COVID-19
Genetics Sudies
DOENÇAS [C]::Infecções::Viroses::Infecções por Vírus de RNA::Infecções por Nidovirales::Infecções por Coronaviridae::Infecções por Coronavirus::COVID-19
03 Saúde e Bem-Estar
Erratum in: Correction: Comparison of DNA extraction methods for COVID-19 host genetics studies. Silva RCD, de Lima SC, Dos Santos Reis WPM, de Magalhães JJF, de Oliveira RN, Rathi B, Kohl A, Bezerra MAC, Pena L. PLoS One. 2024 Nov 15;19(11):e0314215. doi: 10.1371/journal.pone.0314215. eCollection 2024.
This work was supported with resources from the FIOCRUZ Inova Program (Inova COVID19), grant No. 48401971435247.
The coronavirus disease 2019 (COVID-19) pandemic has resulted in global shortages in supplies for diagnostic tests, especially in the developing world. Risk factors for COVID-19 severity include pre-existing comorbidities, older age and male sex, but other variables are likely play a role in disease outcome. There is indeed increasing evidence that supports the role of host genetics in the predisposition to COVID-19 outcomes. The identification of genetic factors associated with the course of SARS-CoV-2 infections relies on DNA extraction methods. This study compared three DNA extraction methods (Chelex®100 resin, phenol-chloroform and the QIAamp DNA extraction kit) for COVID-19 host genetic studies using nasopharyngeal samples from patients. The methods were compared regarding number of required steps for execution, sample handling time, quality and quantity of the extracted material and application in genetic studies. The Chelex®100 method was found to be cheapest (33 and 13 times cheaper than the commercial kit and phenol-chloroform, respectively), give the highest DNA yield (306 and 69 times higher than the commercial kit and phenol-chloroform, respectively), with the least handling steps while providing adequate DNA quality for downstream applications. Together, our results show that the Chelex®100 resin is an inexpensive, safe, simple, fast, and suitable method for DNA extraction of nasopharyngeal samples from COVID-19 patients for genetics studies. This is particularly relevant in developing countries where cost and handling are critical steps in material processing.
2026-01-22T17:02:43Z
2023
journal article
SILVA, Ronaldo Celerino da et al. Comparison of DNA extraction methods for COVID-19 host genetics studies. PLOS One, v. 18, n. 10, p. 1-14, Oct. 2023.
1932-6203
https://arca.fiocruz.br/handle/icict/74707
10.1371/journal.pone.0287551
eng
open access
application/pdf
application/pdf
Public Library of Science